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biotin conjugated horse  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotin conjugated horse
    Biotin Conjugated Horse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 7576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotin+conjugated+horse+anti+mouse+igg/Biotinylated+Horse+Anti-Mouse+IgG+Antibody/pm42000019-134-0-6
    Average 96 stars, based on 7576 article reviews
    biotin conjugated horse - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: Time-Course of Changes in Astrocyte Endfeet Damage in the Hippocampus Following Experimental Ischemia and Reperfusion Injury
    Article Snippet: The sections were then exposed to mouse anti-NeuN antibody (diluted 1:1150; Chemicon International, MAB377, Temecula, CA, USA) overnight at 4○C. .. Subsequently, they were incubated with a biotin-conjugated horse anti-mouse IgG (diluted 1:230; Vector Laboratories, BA-2000) for 2 h at room temperature. .. The signal enhancement was achieved using an avidin-biotin complex (diluted 1:230; Vector Laboratories, PK-6100).

    Article Title: Molecular response of nasal mucosa to therapeutic exposure to broad-band ultraviolet radiation
    Article Snippet: The slides were washed 3 times in phosphate-buffered saline (PBS) for 5 min. each and incubated with proteinase K (10 μg/ml in PBS) at 37°C for 10 min. After 3 washes for 5 min. each in PBS sections were incubated with 5% horse (blocking) serum for 30 min. at 23°C and incubated overnight at 4°C with monoclonal antibody specific for CPDs (clone KTM53, Kamiya Biomedical, Seattle, WA, USA) diluted 1:10,000 in PBS. .. After primary antibody incubation, the slides were washed 3 times for 3 min. in PBS at 23°C and incubated with biotin-conjugated horse anti-mouse IgG (Vector Laboratories, Burlingame, CA, USA) diluted 1:500. .. The slides were then washed 3 times for 3 min. each in PBS, incubated in Vector Elite ABC peroxidase for 30 min. and washed 3 times for 3 min. in PBS at 23°C.

    Article Title: Morphological analysis of patchy thickening and reddish discoloration of active hair growth areas in the skin of New Zealand White rabbits
    Article Snippet: To enhance immunoreactivities for PCNA staining, the sections were heated in CC 1 buffer (950-124, Roche Diagnostics K.K.). .. After incubation with anti-PCNA or anti-collagen type 1 antibodies, sections were treated with a secondary antibody, biotin-conjugated horse anti-mouse IgG (BA-2001, Vector Laboratories, Burlingame, CA, USA) or biotin-conjugated horse anti-goat IgG (BA-9500, Vector Laboratories). .. Then the immunoreaction was visualized by peroxidase-diaminobenzidine reaction using an iVIEW DAB research kit (760-093, Roche Diagnostics K.K.).

    Article Title: Targeted deletion of miR-132/-212 impairs memory and alters the hippocampal transcriptome
    Article Snippet: Sections incubated in 0.3% hydrogen peroxide/PBS for 20 min to eliminate endogenous peroxidase activity and subsequently blocked in 10% normal goat serum or normal horse serum in PBS for 1 h. Sections were then incubated with primary antibody overnight at 4°C using mouse polyclonal anti-MASH1 (1:50 dilution; catalog 556604; BD Pharmingen) or rabbit polyclonal anti-Syntaxin 1a (1:500; dilution; catalog 41753; Abcam). .. The following day, sections were washed and incubated with biotin-conjugated horse anti-mouse IgG or goat anti-rabbit IgG secondary antibody (1:500 dilutions; Vector Laboratories) for 2 h. The ABC labeling (Vector Labs) followed by DAB development (Vector Labs) was used to visualize the signal. .. Sections were mounted on gelatin-coated slides with Permount Mounting Medium (Fisher Chemical).

    Article Title: Local innate responses and protective immunity after intradermal immunization with bovine viral diarrhea virus E2 protein formulated with a combination adjuvant in cattle.
    Article Snippet: Bovine viral diarrhea virus (BVDV) is one of the most serious pathogens in cattle.. Recently, we developed a novel adjuvant platform (TriAdj) that includes a toll-like receptor 3 agonist, poly (I:C); an innate defense regulatory peptide; and water-soluble polymer, poly[di(sodiumcarboxylatoethylphenoxy)]-phosphazene (PCEP).. To develop a needle-free intradermal (ID) subunit vaccine, the BVDV type-2 E2 protein was formulated with TriAdj, and immune protection was evaluated in calves against a BVDV-2 strain.

    Labeling:

    Article Title: Targeted deletion of miR-132/-212 impairs memory and alters the hippocampal transcriptome
    Article Snippet: Sections incubated in 0.3% hydrogen peroxide/PBS for 20 min to eliminate endogenous peroxidase activity and subsequently blocked in 10% normal goat serum or normal horse serum in PBS for 1 h. Sections were then incubated with primary antibody overnight at 4°C using mouse polyclonal anti-MASH1 (1:50 dilution; catalog 556604; BD Pharmingen) or rabbit polyclonal anti-Syntaxin 1a (1:500; dilution; catalog 41753; Abcam). .. The following day, sections were washed and incubated with biotin-conjugated horse anti-mouse IgG or goat anti-rabbit IgG secondary antibody (1:500 dilutions; Vector Laboratories) for 2 h. The ABC labeling (Vector Labs) followed by DAB development (Vector Labs) was used to visualize the signal. .. Sections were mounted on gelatin-coated slides with Permount Mounting Medium (Fisher Chemical).



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